Cell death and cell proliferation in mouse submandibular gland during early post-irradiation phase.

Imbalance between apoptosis and proliferation causes late radiation damage of salivary gland in mouse.

Severe xerostomia is a common late radiation consequence, which occurs after irradiation of head and neck malignancies. The aim of the present study was to analyze apoptosis and proliferation and their relationship during the late post-irradiation phase. C57BL/6 mice were locally irradiated in head and neck region with a single dose of 7.5 or 15 Gy and their submandibular glands were collected ...

Apoptosis and expression of AQP5 and TGF-beta in the irradiated rat submandibular gland.

PURPOSE To evaluate the effect of X-ray irradiation on apoptosis and change of expression of aquaporin 5 (AQP5) and transforming growth factor-β(TGF-β) in the rat submandibular gland (SMG). MATERIALS AND METHODS SMGs of 120 male Sprague-Dawley rats were irradiated with a single X-ray dose (3, 10, 20, or 30 Gy). At the early and late post-irradiation phase, apoptosis was measured by the termin...

Cell proliferation is not required for the initiation of early cleft formation in mouse embryonic submandibular epithelium in vitro.

An X-ray irradiation method was employed to analyse the role of cell proliferation in vitro in the cleft formation of mouse embryonic submandibular epithelium at early stages. When the mid 12-day gland was exposed to 200 rad of X-rays, the growth was severely retarded. In contrast, late 12-day and early 13-day glands grew apparently in a normal fashion, as did the control gland, for up to 40 h....

Mechanism of the protective effect of phenylephrine pretreatment against irradiation-induced damage in the submandibular gland

Irradiation is a fundamental treatment modality for head and neck malignancies. However, a significant drawback of irradiation treatment is the irreversible damage to salivary glands in the radiation field. Although the protective effect of phenylephrine pretreatment on salivary glands following irradiation has previously been demonstrated, the exact mechanism remains unclear. In this study, we...

The Adult Mouse Submandibular Gland Stem/Progenitor Cell – its Localization and Fate after Post-irradiation Transplantation